Polyplus-transfection

Product Range: 
  • Specifications:

    If you routinely perform transient gene expression studies and/or gene silencing by RNA interference, the new powerful jetPRIME™ is the reagent for you!

    Transfection with this versatile reagent requires low quantities of nucleic acid and small amounts of reagent. Therefore, jetPRIME™ is both gentle to cells and economical. A simple and convenient protocol ensures that it’s easy to scale up or scale down.

    Features/Benefits:

    • Outstanding DNA transfection efficiency
    • Economical: less DNA and less reagent needed
    • Better cell viability
    • Excellent gene silencing
    • Cotransfection of DNA & siRNA
    • shRNA and miRNA plasmid transfection
    • Convenient protocol

    Specifications:

    Catalog Number Description
    114-01

    jetPRIME kit 0.1 ml of jetPRIME + 5 ml of jetPRIME buffer

    114-07

    jetPRIME kit 0.75 ml of jetPRIME + 40 ml of jetPRIME buffer

    114-15

    jetPRIME kit 1.5 ml of jetPRIME + 2 x (40 ml of jetPRIME buffer)

    114-75

    jetPRIME kit 5 x (1.5 ml of jetPRIME) + 1 x 55 ml of jetPRIME buffer 10x (conc.)

    114-75C

    jetPRIME kit 5 x (1.5 ml of jetPRIME) + 1 x 120 ml of jetPRIME buffer 5x (conc.)

    1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request

    Contact us for a trial size of jetPRIME™ DNA & siRNA transfection reagent. Special offer with your first order of jetPRIME™!

    For more information on jetPRIME™, click here.

  • Specifications:

    INTERFERin™ siRNA transfection reagent achieves over 90 % silencing efficiency at 1 nM siRNA in a wide variety of adherent and primary cells with excellent cell viability. Using low siRNA concentrations avoids unwanted toxic and off-target (non-specific) effects observed when transfecting siRNA at higher concentrations, resulting in more robust data.

    Features/Benefits:

    • Ready-to-use
    • Compatible with serum and antibiotics
    • Easy standard protocol
    • Gentle mode of action for more robust data and excellent cell viability

    Specifications:

    CATALOG # DESCRIPTION NUMBER OF TRANSFECTIONS
    409-01 INTERFERin™ (0.1ml) 50-100 siRNA transfections in 24-well plates (1 nM siRNA concentration)
    409-10 INTERFERin™ (1ml) 500-1000 siRNA transfections in 24-well plates (1 nM siRNA concentration)
    409-50 INTERFERin™ (5 x 1ml) 5000-10000 siRNA transfections in 24-well plates (1 nM siRNA concentration)

    1 ml of INTERFERin™ is sufficient to perform 500-1000 transfections in 24-well plates.

    For more information on siRNA in vitro - INTERFERin™, click here.

  • Specifications:

    jetPEI™ transfection reagent is particularly well suited for automated or manual HTS (High Throughput Screening) as transfection with jetPEI™ is exceptionally efficient and reproducible. A distinctive linear polyethylenimine (PEI) derivative, jetPEI™ gently facilitates intracellular DNA delivery for high performance. 

    Three HTS protocols are available depending on the experiment: forward, batch or reverse.

    Figure: HEK-293 cells expressing GFP after transfection using jetPEI™.

    Features/Benefits:

    • Fast and efficient methods to transfect cells for HTS
    • Compatible with serum and antibiotics
    • Robust jetPEI/DNA complexes
    • Exceptionally reproducible results
    • Well-suited for automated approaches

    Specifications:

    CATALOG # Amount of reagent Amount of NaCl solution
    101-01N 0.1 mL 5mL
    101-10N 1 mL 50 mL
    101-10 1 mL -
    101-40N 4 x 1mL 4 x 50 mL
    101-40 4 x 1 mL -
    101B-010N 10 mL 2 x 250 mL
    101B-010 10 mL -

    One ml of jetPEI™ is sufficient to perform 2 000 transfections in 96-well plates.

    For more information on DNA for HTS application - jetPEI™, click here.

     

  • Specifications:

    NEW! INTERFERin™-HTS is a new generation siRNA transfection reagent especially developed for high throughput screening (HTS) applications providing great silencing efficiency, excellent reproducibility and high cell viability with very low amounts of reagent.

    INTERFERin™-HTS is cost-effective, easy to handle, compatible with serum and antibiotics, and comes with reverse and forward protocols for 96- and 384-well plates.

    Figure 4. siRNA transfection with INTERFERin™-HTS gives higher silencing efficiency than competitor reagents. HeLa cells were transfected in 96-well plates with 10 nM siRNA duplexes targeting lamin A/C following a reverse transfection protocol according to the manufacturer‘s recommendations. Lamin A/C expression was measured 24 hours after transfection by branched DNA assay and normalized to GAPDH expression.

    Features:

    • Great silencing from 10 to 50 nM siRNA
    • Designed for automated procedures
    • Reproducible at all levels
    • Small volume of reagent per well, cost effective

    Specifications

    Catalog Number Amount of reagent
    410-002 0.2 mL
    410-015 1.5 mL
    410-060 4 x 1.5 m

    1.5 mL of INTERFERin™-HTS is sufficient to transfect 200-300 plates in (96-well)

    For more information on INTERFERin™-HTS, click here.

  • Specifications:

    Non-viral gene delivery to animals is easy with in vivo-jetPEI™ transfection reagent. Simply mix your DNA (or siRNA) with this reagent and administer to animals for gene expression in multiple tissues.

    With in vivo-jetPEI™, you can also deliver genes by different modes into different species based on your needs (even humans in GMP conditions). You'll avoid experimental and regulatory hassles by choosing in vivo-jetPEI™ in place of virus for gene delivery.

    This reagent can be made under GMP conditions and customized to target specific cells.

    Figure : Non-invasive bioluminescence of a living mouse following systemic delivery of in vivo-jetPEI™/DNA complexes.

    Features:

    • Successful delivery of DNA, siRNA and oligonucleotides in vivo
    • Multiple modes of administration in many species
    • RNAi with in vivo-jetPEI™
    • No detectable inflammatory response
    • Currently used in several phase I-II clinical trials
    • Reproducible results

    Specifications:

    CATALOG # Amount of reagent Amount of glucose solution
    201-10G 0.1ml 10 mL
    201-50G 0.5 mL 2 x 10 mL

    0.1ml of in vivo-jetPEI™ is sufficient to perform up to 20 intravenous injections in mouse with 50 µg of DNA.

    For more information on in vivo-jetPEI™, click here.

  • Specifications:

    PULSin™ is an innovative reagent for the highly efficient transfer of functional proteins to the cytoplasm of living cells. With PULSin™, you can target intracellular proteins in living cells with antibodies without fixation, eliminating the time and effort of fixing cells.

    PULSin™ also has advantages over DNA transfection because the amount and timing of exogenous proteins in cells can be controlled. With this precise control even lethal proteins can be studied.

    Simple and fast protocol

    Features:

    • Effective and robust delivery into the cytoplasm
    • Delivery to a wide variety of cells including primary cells, with up to 98% efficiency
    • Highly efficient transfer
    • Easy to use (Simply add PULSin™ to the protein, incubate, and apply to cells)
    • Easily perform real time function blocking and protein localization studies using antibodies.

    Specifications:

    Catalog # Reagent Number of Reactions
    501-01 PULSin™ (0.1ml)* 6 reactions in 6-well plates
    501-04 PULSin™ (0.4ml)* 24 reactions in 6-well plates
    501-16 PULSin™ (0.4ml)* 96 reactions in 6-well plates

    0.4 ml of PULSin™ protein delivery reagent is sufficient for 24 reactions in 6-well plates.

    For more information on PULSin™, click here.

  • Specifications:



     

    FectoPROTM gives amazing antibody production yields in suspension CHO cells.
    Cells were seeded at 1 x 106 cells/ml in 30ml of FreestyleTM CHO Expression Medium and transfected with FectoPROTM (0.8μg DNA/ml) or FreeStyleTM MAX Reagent (1.25μg DNA/,ml)
    following recommended protocols for the respective reagents. Recombinant mouse lgG production yield was assayed 1 to 6 days after transfection using protein G biosensor (fortéBIO® octet RED96 system).

    Data kindly provided by ProteoGenix SAS (France)

    FectoPROTM gives high transfection efficiency in suspension CHO cells.
    FreeStyleTM CHO-S cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO Expression Medium and transfected with FectoPROTM + FectoPROTM Booster of FreeStyleTM MAX Reagent using 0.4μg/ml and 1.25μg/,l of a GFP expressing plasmid, respectively. GFP expression was assayed 24 h after transfection using fluorescence microscopy.

    FectoPROTM gives superior protein prodcuction yields in suspension CHO and HEK-293 cells.
    FreeStyleTM CHO-S and HEK-293F cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO or 293 Expression Medium and transfected with FectioPROTM + FectoPROTM Booster (0.4μg DNA/ml), FreeStyle TM MAx Reagent (1.25μg DNA/ml) or PEIproTM (1μg DNA/ml) following recommended protocols for the respective reagents. IgG3-Fc (34kDa) production was assayed 72 h (CHO cells) and 120 h (HEK-293 cells) after transfection by protein G affinity quantification (HPLC) and Western Blot. Standard corresponds to purified IgG3-Fc (34kDa).

    FectoPROTM HEK-293F cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM 293 Expression Medium and transfected with FectoPROTM + FectoPROTM Booster (0.5 μg DNA/,l) following the standards protocol. IgG3-Fc production was assayed at different day by protein G affinity quantification (HPLC). Similar results are obtained with FreeStyleTM CHO-S (data not shown).

    FectoPROTM is easily scalable and reproducible in CHO cells.
    FreeStyleTM CHO-S cells were seeded at 1 x 106 cells/ml in 30ml, 100ml, 300ml or 1L of FreeStyleTM CHO Expression Medium and transfected with FectoPROTM + FectoPROTM Booster (0.5μg DNA/ml). At day 1 to 6, cell viability was assayed using Trypan blue exclusion assay (A) and IgG3-Fc production was assayed by protein G affinity quantification (HPLC) (B) and Western Blot (C). Standard (C) corresponds to purified IgG3-Fc (34 kDa).

    Suspension CHO cells were seeded at 1 x 106 cells/ml in 30 ml of CHO medium and transfected using 3 different lots of FectoPROTM (CCIII 078, CCIII 104 or CCIII 106) following the standard protocol. 72 h after transfection, IgG3-Fc production was assayed by protein G affinity quantification. Similar results are obtained on suspension HEK-2933 cells (data not shown).

    FectoPROTM gives superior protein production yields in suspension CHO cells grown in different media. CHO cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO Expression Medium, CD CHO Medium or Pro-CHOTM 4 Medium and transfected with FectoPROTM + FectPROTM Booster or FreeStyleTM MAX Reagent following recommended protocols for the respective reagents. IgG3-Fc production was assayed 72 h after transfection by protein G affinity quantification (HPLC).

  • Specifications:

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Description: 
Polyplus-transfection is focused on developing reagents for intracellular delivery of biomolecules. Its expertise includes in vitro and in vivo delivery of genes, oligonucleotides, siRNA and proteins. With over half its revenues reinvested into R&D, Polyplus-transfection covers all aspects including the physical chemistry, cell biology and pharmacology of carriers. This leads to constant improvement of existing solutions as well as original compounds at the forefront of delivery science. Catalog
Country: 
France