Rac1 Regulation ASCB 2015 Abstract

Temporal regulation of phosphotyrosine-modified Rac1 in response to epidermal growth factor stimulation

Abstract

The aim of this study was to develop an assay sensitive enough to detect endogenous pY‐modified Rac1 uponstimulation by EGF. The IP assay was used in conjunction with a Rac1 activation assay to follow temporal changesin endogenous Rac1 activation and tyrosyl phosphorylation in response to EGF stimulation of HeLa and A431 cells. Rac1 activation, monitored by PAK‐binding, followed a predicted time course in which...
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Link to citation: 

Law A., Hong S., Horita H. and Middleton K. 2015. Temporal regulation of phosphotyrosine-modified Rac1 in response to epidermal growth factor stimulation. Mol. Biol. Cell. ASCB Abstract Dec. B1230 / P2126. 

Products used in this citation:

  •     Rac1 Pulldown Activation Assay Kit (Cat. # BK035)
  •     Anti-Phosphotyrosine HRP conjugated (Cat. # APY03-HRP)
  •     Anti-Phosphotyrosine Affinity Beads (Cat.# APY03-beads