Cell Emigre

Manufacturer: 
Specifications: 

A quick & quantitative assay for determining linear cell migration rate.
It is designed for use with attachment-dependent mammalian cells.

What can Cell Émigré be used for?

  • Cell Émigré is designed to measure the linear migration rate of attachment-dependent mammalian cells.
  • Migration rate responses can be readily compared across different cell types. The assay provides a rapid means of evaluating and comparing the effect of chemical or biological treatments such as chemical agents, proteins, peptides, antibodies or genetically modified cells.

What are the advantages of Cell Émigré?

1. AN EASY-TO-USE ASSAY

The end-user supplies a cell sample (of freshly detached cells in fluid suspension) to a microfluidic ‘chip’. The chip then automatically distributes cells to microchannel arrays – ready for attachment & migration. No pumps or external fluid connections are required; each chip contains a reservoir for culture medium which is filled during the initial cell addition step.

2. MEASURES THE RATE OF LINEAR CELL MIGRATION – NO MORE GOING IN CIRCLES!

Existing 2D-migration assays often attempt to measure ‘random-walk’ cell migration – a challenging task! Cell Émigré takes the innovative approach of quantifying migration within an array of linear microchannels. Each cell under assay is constrained to a linear migration path through contact-guided migration along a microchannel wall.

3. RAPID MIGRATION QUANTIFICATION

Cells begin migrating within Cell Émigré aligned to a common START position; a cells displacement from this position indicates its linear migration distance. There is no need for video recording or computer image analysis.

Each microchannel in the array contains a parallel measurement scale calibrated in microns. Cell migration can be rapidly & easily quantified (to the nearest 50μm) by visually comparing the alignment of a cell within the microchannel with this integrated measurement scale (using x40-x100 microscopic examination). Migration can be quantified at any time point during an assay.

Results may be expressed as a migration rate: microns per hour (μm/h).

4. REDUCED REAGENT CONSUMPTION

Achieved through the use of microfluidic technology. A single chip containing 3x assays consumes <350μl of fluid when loaded. This reduces costs in terms of cells and reagents.

Product and Supporting Material

For more information, please visit:
http://www.biocolor.co.uk/index.php/assay-kits/cell-emigre-cell-migration-assay