ArcticZymes Technologies

Product Range: 
  • Specifications:

    Born from the unique conditions in the Arctic and their labs in Tromsø (Norway), ArcticZymes have been developing and producing cold-adapted enzymes for more than 30 years. Their high-quality enzymes are an integral part of molecular research and diagnostics, either as stand-alone enzymes or as components of kits. In therapeutics such as gene therapy and vaccine production their enzymes aide the optimisation of manufacturing processes.

     

    Catalog

  • Specifications:

    SAN High Quality (Bioprocessing grade) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.

    Salt is an important component of various purification schemes. The presence of salt can minimize aggregation, increase target solubility and improve target yield. High salt enables contaminating DNA to dissociate from associated proteins and become available for degradation. SAN High Quality is highly compatible with the use of high salt conditions.

    More details here.

  • Specifications:

    M-SAN HQ is the latest addition to our high salt tolerance nucleases portfolio. The biochemical properties of the SAN family of nucleases make them ideal for multiple bioprocessing and biomanufacturing workflows.

    More details here.

    M-SAN HQ has been developed to offer the optimal solution for removal of nucleic acids at the near-physiological conditions used in many bioprocessing and biomanufacturing workflows. This novel, nonspecific endonuclease is active over a broad pH range and displays optimum activity at salt concentrations around 175 mM.

  • Specifications:

    Removal of nucleic acids may improve workflows during protein purification, both for laboratory sample preparation and industrial bioprocessing. HL-SAN is a nonspecific endonuclease with optimum activity at high salt concentrations. HL-SAN is active in a variety of buffers and can be easily inactivated by treatment with a reducing agent. These features make HL-SAN particularly useful in the purification of proteins and removal of DNA and RNA from molecular biology reagents.

    Nucleic acids, and especially genomic DNA, often pose a problem in purification of DNA-binding proteins as they interfere with purification, downstream analysis or applications.
    As most nucleases are inhibited by high concentrations of salt, removal of DNA is difficult, since NaCl is used to dissociate DNA from proteins.

    The high salt-tolerance and easy removal makes HL-SAN beneficial to use in protein purification schemes, especially in combination with IMAC (Immobilized metal affinity chromatography) media used for purification of poly-His-tagged proteins both in conventional chromatography or high-throughput settings.

    More details here.

  • Specifications:

    IsoPol™ DNA Polymerase is active at ambient temperatures.

    It exhibits 5’-3’ polymerase activity and lacks 3’-5’ exonuclease activity. The 5’-3’ exonuclease domain is truncated, thus the enzyme also lacks 5’-3’ exonuclease activity. IsoPol™ DNA Polymerase has excellent processivity and strong stand-displacement activity.

    In addition, it can be heat inactivated at temperatures above 50°C.

    More details here.

  • Specifications:

    IsoPol™ SD+ is a DNA polymerase with stronger strand displacement and higher salt tolerance, compared to ArcticZymes first IsoPol™.

    More details here.
     

  • Specifications:

    IsoPol™ BST+ is a heat-tolerant Bst polymerase (large fragment) with enhanced strand-displacement activity. IsoPol™ BST+ is active from 25 to 65°C. It is lacking 5’-3’- and 3’-5’-exonuclease activity.

    More details here.

  • Specifications:

    Cod Uracil-DNA Glycosylase (Cod UNG) from Atlantic cod is completely and irreversibly inactivated by moderate heat treatment. The enzyme is produced in a recombinant E. coli (ung–) strain that contains a modified Cod UNG gene.

    More details here.

  • Specifications:

    Double-strand specific DNases (dsDNases) are unique double-strand specific endonucleases. As they do not digest ssDNA or RNA, they can be used to specifically remove dsDNA in the presence of other nucleic acids. The enzymes are heat-labile, which makes them ideal for applications where the DNase have to be inactivated. There are two versions of this enzyme available, dsDNase and HL-dsDNase.

    More details here.

  • Specifications:

    Shrimp Alkaline Phosphatase (SAP) – still the golden standard and the first heat-labile alkaline phosphatase on the market. It is a multipurpose alkaline phosphatase that can be fully inactivated by a short heat treatment.

    More details here.

  • Specifications:

    HL-ExoI is a 3’-5’ exonuclease, specific for single stranded DNA. HL-ExoI is active at temperatures ≥25°C, and inactivated by 1 min incubation at 80°C, or 15 min at 60°C.

    More details here.

  • Specifications:

    ArcticZymes Proteinase is an unspecific endopeptidase originating from an Arctic marine microbial source. It has broad substrate specificity and is easy to inactivate after use.

    Histones and other proteins are known to protect nucleic acids from interacting optimally with other DNA binding proteins and enzymes. ArcticZymes Proteinase is ideally suited for transforming chromatin and other dense nucleic acids to naked DNA. The enzyme is easy to heat-inactivate. This allows thermal inactivation at temperatures allowing RNA integrity as well as avoiding dissociation of dsDNA.

    More details here.

  • Specifications:

    T4 DNA Ligase is an ATP and Mg2+ dependent dsDNA ligase which catalyses the formation of a phosphodiester bond between 3’-hydroxyl and 5’-phosphate termini in duplex DNA, duplex RNA and some DNA/RNA hybrids. T4 DNA Ligase is active on both blunt-end and cohesive-end substrates.

    This is a high-quality (commercial grade) version of the T4 DNA Ligase. T4 DNA Ligase is recombinantly produced in E. coli. ArcticZymes’ T4 DNA Ligase is extensively tested for contaminating DNase and RNase activities as well as residual host-cell gDNA.

    More details here.

Description: 
Born from the unique conditions in the Arctic and their labs in Tromsø (Norway), ArcticZymes have been developing and producing cold-adapted enzymes for more than 30 years. Their high-quality enzymes are an integral part of molecular research and diagnostics, either as stand-alone enzymes or as components of kits. In therapeutics such as gene therapy and vaccine production their enzymes aide the optimisation of manufacturing processes.  Catalog
Country: 
Norway