Cytoskeleton
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Specifications:
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Specifications:
Live-cell Actin and Tubulin Fluorescent Probes
Live-cell imaging probes developed by Spirochrome are cell-permeable compounds which stain microtubules (SiR-Tubulin) and F-actin (SiR-Actin) in living cells. These probes were recently introduced in a landmark paper published in Nature Methods
and recently featured on the cover of the Journal of Biological Chemistry. A selection of researcher-provided images, videos, and citations are listed below. For more images, videos, and product details visit our website.Cardiac myocytes
Movie of newborn mouse primary cardiac myocytes stained with SiR-actin. High speed (50fps) confocal imaging. Courtesy of Adam Kwiatkowski and Simon Watkins, Department of Cell Biology and Center for Biologic Imaging, University of Pittsburgh.HeLa cells
Movie of HeLa cell expressing mcherry-H2B (red) stained with SiR-tubulin (green). Data collected by confocal imaging. Courtesy of Daniel Gerlich and Claudia Blaukopf, Institute of Molecular Biotechnology, Vienna.
Goldfish retinal bipolar cell
3-D projection of a single isolated goldfish retinal bipolar cell stained with SiR-tubulin. The color spectrum represents depth. Microtubules can be seen to extend from the tips of the dendrites (top) into the axon and down into the giant synaptic terminal (bottom). Courtesy of Justin W. Taraska, Laboratory of Cellular Biophysics, NIH, Bethesda.
MCF10A Cells (3D culture)
MCF10A cells expressing H2B-GFP (blue) in Matrigel stained with SiR-actin (red). Image taken on an inverted LSM microscope. Courtesy of Christian Conrad and Katharina Jechow, Heidelberg.
HUVEC Cells
HUVEC Cells (fixed), stained with SiR-actin and imaged by confocal microscopy.
3D reconstruction from Z-stack. Courtesy of Urim Retkoceri and Steffen Dietzel, Ludwig-Maximilians Universitat, Munchen.
Rat hippocampal neurons
STED image of cultured rat hippocampal neurons stained with SiR-actin. Actin rings (stripes) with 180 nm periodicity can be seen. Courtesy Of Elisa D'Este, MPI Biophysical Chemistry, Göttingen.Featured Papers
"Direct Interaction of CaVβ with Actin Up-regulates L-type Calcium Currents in HL-1 Cardiomyocytes"; G. Stölting, R.C. Oliveira, R. Guzman, E. Miranda-Laferte, R. Conrad, N. Jordan, S. Schmidt, J. Hendriks, T. Gensch & P. Hidalgo; J. Biol. Chem. 290, 4561-4572, 2015.
"Fluorogenic probes for live-cell imaging of the cytoskeleton"; G. Lukinavičius, L.Reymond, E. D'Este, A. Masharina, F. Göttfert, H. Ta, A. Güther, M. Fournier, S. Rizzo, H. Waldmann, C. Blaukopf, C. Sommer, D. W. Gerlich, H.-D. Arndt, S. W. Hell & K. Johnsson; Nature Methods 11, 731-733, 2014.
"STED Nanoscopy Reveals the Ubiquity of Subcortical Cytoskeleton Periodicity in Living Neurons"; E. D'Este, D. Kamin, F. Göttfert, A. El-Hady, S. W. Hell; Cell Reports , Volume 10 , Issue 8 , 1246 - 1251, 2015.
"A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins"; G. Lukinavičius, K. Umezawa, N. Olivier, A. Honigmann, G. Yang, T. Plass, V. Mueller, L. Reymond, I. R. Corrêa Jr, Z. Luo, C. Schultz, E. A. Lemke, P. Heppenstall, C. Eggeling, S. Manley & K. Johnsson; Nature Chemistry 5, 132-139, 2013.
"Dynamic actin filaments control the mechanical behavior of the human red blood cell membrane"; D. S. Gokhin, R. B. Nowak, J. A. Khoory, A. de la Piedra, I. C. Ghiran and V. M. Fowler; Mol. Biol. Cell; February 25, 2015.
"A cleavable cytolysin-neuropeptide Y bioconjugate enables specific drug delivery and demonstrates intracellular mode of action"; V. M. Ahrens, K. B. Kostelnik, R. Rennert, D. Böhme, S. Kalkhof, D. Kosel, L. Weber, M. von Bergen and A. G. Beck-Sickinger; J. Control. Release; 209:170-178, 2015.
"Red Si-rhodamine drug conjugates enable imaging in GFP cells"; E. Kim, K. S. Yang, R. J. Giedt and R. Weissleder; Chem. Commun., 50, 4504-4507, 2014.
"A marginal band of microtubules transports and organizes mitochondria in retinal bipolar synaptic terminals"; M. Graffe, D. Zenisek, and J. Taraska; J. Gen Physiol. Vol. 146 No.1: 109-117, 2015.
Application Notes
"A Bright Dye for Live-Cell STED Microscopy"; S. Pitsch, I. Köster.
