Nacalai Tesque, Inc
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Specifications:
Features
- Only 10 minutes with 400 V
- High transfer efficiency of proteins on western blot membrane
- Works well in conventional Laemmli running buffer and sample buffer
- 17-well gel is usable with multichannel pipet for consistent loading
>> Click here for the video of Bullet PAGE One Precast Gel vs Laemmli gel 16x
Performance Comparison
Bullet PAGE One Precast Gel can produce an excellent separation image with the shortest electrophoresis.Product Name Bullet PAGE One Company A Company B Gel ％ 5 - 15％ 4 - 15％ 4 - 12％ Product No. 13079-84 - - Electrophoresis Time 11 min. 33 min. 50 min. Electrophoresis
Images
<Electrophoresis condition>
Sample: Protein Markers(10x)(Prod No. 29458-24), 5 µl Gel Staining: CBB Stain One (Prod No. 04543) Voltage Constant: Bullet PAGE One: 400V, Company A and Company B: 200V Comparison of Protein Transfer Efficiency
Bullet PAGE One Precast Gel indicates obviously higher protein transfer efficiency than company A’s, even though blotting time was only 10 min.
<Sample>
① Protein Ladder One(Triple-color)(Product No. ： 09547-74)
② Protein Markers(3x) 2 µl added
④ Protein Markers(1x) 2 µl added
⑤ Protein Markers(1/3) 2 µl added
<Transfer>
Use Semi ‐ dry Blotting Buffer Solution for Western Blotting (Product No.: 30650-31)
(Transfer time : 10 min. and 20 min. 10V)
<PVDF membrane staining>
Stain PVDF membrane with CBB Stain One (Product No. ： 04543).Gel Types
Gel Gradient Gel Single Percentage Gel Gel ％ 5 - 11% 5 - 15% 6% 8% Product No. 13 well
17 well13077-04
13078-9413079-84
13080-4413081-34
13082-2413083-14
13084-04Electrophoresis Images 
Product Specification
Glass Plate Size: W100mm x H80mm x T3.2mm Gel Size: W80mm x H60mm x T1.0mm Sample Well Configuration / Maximum Load Volume: 13-well / 40 µl, 17-well / 28 µl -
Specifications:
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Specifications:
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Specifications:
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