Nacalai Tesque, Inc

Features

• Only 10 minutes with 400 V
• High transfer efficiency of proteins on western blot membrane
• Works well in conventional Laemmli running buffer and sample buffer
• 17-well gel is usable with multichannel pipet for consistent loading

>> Click here for the video of Bullet PAGE One Precast Gel vs Laemmli gel 16x

Performance Comparison
Bullet PAGE One Precast Gel can produce an excellent separation image with the shortest electrophoresis.

Product Name	Bullet PAGE One	Company A	Company B
Gel ％	5 - 15％	4 - 15％	4 - 12％
Product No.	13079-84	-	-
Electrophoresis Time	11 min.	33 min.	50 min.
Electrophoresis Images

<Electrophoresis condition>

Comparison of Protein Transfer Efficiency

Bullet PAGE One Precast Gel indicates obviously higher protein transfer efficiency than company A’s, even though blotting time was only 10 min.

<Sample>
① Protein Ladder One(Triple-color)(Product No. ： 09547-74)
② Protein Markers(3x) 2 µl added
④ Protein Markers(1x) 2 µl added
⑤ Protein Markers(1/3) 2 µl added
<Transfer>
Use Semi ‐ dry Blotting Buffer Solution for Western Blotting (Product No.: 30650-31)
(Transfer time : 10 min. and 20 min. 10V)
<PVDF membrane staining>
Stain PVDF membrane with CBB Stain One (Product No. ： 04543).

Gel Types

Gel	Gradient Gel		Single Percentage Gel
Gel ％	5 - 11%	5 - 15%	6%	8%
Product No. 13 well 17 well	13077-04 13078-94	13079-84 13080-44	13081-34 13082-24	13083-14 13084-04
Electrophoresis Images

Product Specification