Toyobo

Product Range: 
  • PCR
    Specifications:

    PCR

    Toyobo has various unique PCR enzymes, kits and PCR related products as shown below. The following tables show the characteristics of each product. Detailed information can be obtained by going to the linked sites.

    PCR Enzyme & Kit

    Enzyme Product Name Fidelity
    (3'→5'
    Exonuclease
    activity)
    Efficiency Velocity
    (Extension
    time)
    Target
    size
    Crude
    sample
    Reverse
    Transcriptase
    activity
    Hot start PCR Product
    Ends
    KOD DNA
    polymerase
    KOD -Plus- ++++
    [80 folds]
    ++ +
    (1 min/kb)
    ~12 kb + - blunt
    KOD -Plus- Neo ++++
    [80]
    ++ +++
    (≤0.5 min/kb)
    ~24 kb + - blant
    KOD FX +++
    [11]
    +++ +
    (1 min/kb)
    ~24 kb +++ - blunt
    KOD FX Neo +++
    [11]
    +++ +++
    (≤0.5 min/kb)
    ~40 kb ++++ - blant
    KOD Dash +
    [3]
    +++ +++
    (≤0.5 min/kb)
    ~18 kb ++ - - mixed
    (blunt &
    3'-dA)
    KOD exo(-) -
    [1]
    +++ +++
    (≤0.5 min/kb)
    ~10 kb ++ - - 3'-dA
    Tth DNA
    polymerase
    rTth DNA Polymerase -
    [1]
    + +
    (1 min/kb)
    ~2 kb +
    (Mn2+)
    - 3'-dA
    RT-PCR Quick Master Mix -
    [1]
    + +
    (1 min/kb)
    ~2 kb + 3'-dA
    Taq DNA
    polymerase
    rTaq DNA polymerase -
    [1]
    + +
    (1 min/kb)
    ~2 kb + - - 3'-dA
    Quick Taq® HS DyeMix -
    [1]
    + +
    (1 min/kb)
    ~4 kb + - 3'-dA
    Blend Taq® +
    [3]
    ++ +
    (1 min/kb)
    ~23 kb ++ - - mixed
    (blunt &
    3'-dA)
    Blend Taq® -Plus- +
    [3]
    ++ +
    (1 min/kb)
    ~23 kb ++ -

    ++++: Best, +++: Excellent or Strong, ++: Good or Moderate, +: satisfactory, -: Not good or minus , : Applicable

    [ ]: Accuracy of each PCR enzyme (reagent) when the accuracy of Taq DNA polymerase is set to 1.

     

    PCR related product

    Product Name Remarks
    Anti-Taq high Anti-Taq DNA polymerase neutralization antibody for hot start
    Deoxyribonucleotide triphosphate (dNTPs) Highly purified dNTPs for PCR
    RNase Inhibitor Highly purified RNase inhibitor for reverse transcription
    TArget Clone™ TA cloning of PCR products having overhanged dA at the 3'-ends. PCR products from Taq DNA polymerase, Blend Taq® [Code No. BTQ-101], Blend Taq® -Plus- [Code No. BTQ-201] or KOD Dash [Code No. LDP-101] are available
    TArget Clone™ -Plus- TA cloning of blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201] or KOD FX [Code No. KFX-101].
    10xA-attachment mix Attachment of dA at the 3' - ends of blunt end PCR products from KOD -Plus- and KOD FX ( For TA cloning )

     

     

    PCR Enzyme & Kit

    High fidelity PCR polymerase

    KOD -Plus-
     

    • The PCR error ratio of KOD -Plus- is approx. 80 times less than Taq DNA polymerase.
    • Hot Start technology results in highly efficient amplification.
    • KOD -Plus- enables the following amplifications (maximum): 21 kb from lambda phage DNA, 12 kb from human genomic DNA, and 7 kb from cDNA.

    KOD -Plus- is based on DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. KOD -Plus- exhibits excellent high PCR fidelity and efficiency. The enzyme solution of KOD -Plus- contains two types of anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activity, thus allowing for Hot Start PCR. KOD -Plus- generates blunt-end PCR products, due to 3'→5' exonuclease (proof-reading) activity.

    Code No. KOD-201 200 U 200 reactions

     

    High fidelity & efficient & fast PCR enzyme

    KOD -Plus- Neo

    • The PCR error ratio of KOD -Plus- Neo is about 80 times less than that of Taq DNA polymerase and is equal to the previous version (KOD -Plus-).
    • Requires only 30 sec/kb for the PCR extension step.
    • "Elongation enhancer" enables greater amplification efficiency and elongation capability (up to 24 kb from human genomic DNA) compared to conventional PCR.

    KOD -Plus- Neo is the improved version of the previous KOD -Plus- (Code No. KOD-201). This polymerase contains a unique "elongation enhancer" that suppresses the "plateau effect" produced by conventional PCR.
    Therefore, this reagent exhibits greater amplification efficiency and elongation capability compared to the previous version of KOD -Plus-.
    Moreover, this enzyme requires only 30 sec/kb for the PCR extension step. This facilitates the long range PCR.

    Code No. KOD-401 200 U , 200 reactions

     

    High success-rate DNA polymerase

    KOD FX

     

    • Effective for the amplification of GC-rich targets and crude samples.
    • Hotstart technology using anti-KOD DNA polymerase antibodies enables highly efficient amplification.
    • KOD FX enables the following amplifications (Maximum): 40kb from lambda phage DNA, 24kb from human genomic DNA, and 13.5kb from cDNA.
    • The PCR error ratio of KOD FX is about 10 times less than that of Taq DNA polymerase.

    KOD FX is based on DNA polymerase from the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. KOD FX results in much greater PCR success based on efficiency and elongation capabilities than KOD -Plus- (Code No. KOD-201) or other Taq-based PCR enzymes. KOD FX enzyme solution contains two types of anti-KOD DNA polymerase antibodies that inhibit polymerase and 3'→5' exonuclease activities, thus allowing for Hot Start PCR. KOD FX generates blunt-end PCR products, due to 3'→5' exonuclease (proof-reading) activity.

    Code No. KFX-101 200 U 200 reactions
       

     

    High efficient & high success-rate PCR enzyme

    KOD FX Neo

    • KOD FX Neo is effective for amplification from crude samples (e.g. biological samples, foodstuffs, soil extract, etc). Various samples or lysates can be used directly as templates.
    • "Elongation enhancer" enables greater amplification efficiency and elongation capability (up to 40 kb from human genomic DNA) compared to conventional PCR. This enzyme is useful for amplifying difficult targets, such as high G/C, A/T, and/or long targets.
    • Various microorganisms (e.g. yeast, fungus, gram-positive bacteria) can be directly used as templates for PCR.
    • The PCR error ratio of KOD FX Neo is about 10 times less than that of Taq DNA polymerase and is equal to the previous version (KOD FX).

    KOD FX Neo is the improved version of the previous KOD FX (Code No. KFX-101). This product contains a unique "elongation enhancer" that suppresses the "plateau effect", enabling greater elongation rates and capabilities.
    The KOD FX Neo enzyme solution contains two types of anti-KOD DNA polymerase antibodies that inhibit the polymerase and 3'→5' exonuclease activities, thus allowing for Hot Start PCR. KOD FX Neo is a highly efficient enzyme that is tolerant of PCR inhibitors in crude samples.

    Code No. KFX-201 200 U , 200 reactions

     

    High Speed & Efficient DNA polymerase

    KOD Dash

     

    • Shows greater elongation velocity than Taq DNA polymerase (2 folds) and Pfu DNA polymerase (6 folds) due to the property of KOD DNA polymerase.
    • The PCR error ratio of this enzyme mixture is approximately 3 to 4 times less than that of Taq DNA polymerase.
    • Effective for the amplification of various targets from a small template amount.

    KOD Dash is a highly efficient DNA polymerase mixture developed based on the Barns' method. This method uses a DNA polymerase which lacked a 3'→5' exonuclease (proofreading) activity and a small amount of an archaeal DNA polymerase with proofreading activity. In the reagent, the 3'→5' exonuclease activity-minus mutant <KOD EXO(-)> of KOD DNA polymerase and KOD DNA polymerase are used. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of a polymerase reaction, PCR with this mixed enzyme solution enables highly efficient amplification. KOD Dash generates dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA cloning method.

    Code No. LDP-101 250 U 200 reactions

     

    High Speed DNA polymerase

    KOD exo (-)

    • Shows greater elongation velocity than Taq DNA polymerase (2 folds) and Pfu DNA polymerase (6 folds) due to the property of KOD DNA polymerase.
    • The PCR error ratio of this enzyme mixture is approximately equal to that of Taq DNA polymerase.
    • This enzyme is effective for the amplification of various targets from a small template amount.

    KOD exo (-) is a 3'→5' exonuclease minus mutant developed based on KOD DNA polymerase from a hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. KOD exo (-) shows excellent elongation velocity.

     

    DNA polymerase having reverse transcriptase activity

    rTth DNA polymerase

    • Exhibits reverse transcriptase activity in addition to DNA polymerase activity in the presence of Mn2+ ions.

    rTth DNA polymerase is a recombinant DNA polymerase derived from the thermophilic bacteria Thermus thermophilus (Tth) HB8. This polymerase exhibits reverse transcriptase activity in addition to DNA polymerase activity in the presence of Mn2+ ions.Therefore, this enzyme enables “one-step RT-PCR” including reverse transcription and PCR steps.

    Code No. TTH-301 250 U 100-200 reactions

     

    One-step RT-PCR Master Mix

    RT-PCR Quick Master Mix

     

    • Suitable for high-throughput RT-PCR.
    • This kit has the advantage of amplifying targets with complex conformations and GC-rich content.
    • Hot-start technology using anti-Tth DNA polymerase antibodies enables highly efficient and specific amplification.

    RT-PCR Quick Master Mix provides a 2 × Master Mix for RT-PCR using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in addition to DNA polymerase activity in the presence of Mn2+ ions. Therefore, this system enables “one-step RT-PCR” including reverse transcription and PCR steps. This kit is suitable for high-throughput RT-PCR, and decreases contamination risks.

    Code No. PCR-311 50 reactions

     

    Standard PCR Enzyme

    rTaq DNA polymerase

    Code No. TAP-201 250 U 100-200 reactions
    rTaq DNA polymerase is a recombinant DNA polymerase derived from the thermophilic bacteria Thermus aquaticus (Taq) YT-1.

     

    Taq DNA polymerase (Dye-premix & Hot-start version)

    Quick Taq® HS DyeMix

    • The hot start technology enables efficient greater PCR performance than conventional Taq DNA polymerase.
    • The master mix is stable for at least three months at 4ºC. No decrease in reaction efficiency is observed following 30 freeze-thaw cycles.

    Quick Taq® HS DyeMix is a Taq-based 2 × master mix PCR reagent that contains an electrophoresis dye (BPB; bromophenol blue) and anti-Taq antibodies for hot start PCR. This reagent contains all components for PCR except primers and template DNA. This reagent shows specific and efficient amplification. The amplified products can be directly loaded in the wells of an agarose or acrylamide gel.

    Code No DTM-101 100 reactions

     

    High Efficient Taq DNA polymerase

    Blend Taq® / Blend Taq® -Plus-

    • The elongation ability of this enzyme is much greater than that of the normal Taq DNA polymerase.
    • Hot Start technology using anti-Taq DNA polymerase antibodies results in highly efficient amplification. <Blend Taq® (Code No. BTQ-101) does not use hot start>
    • The PCR error ratio of this enzyme is approximately 3-4 times less than that of Taq DNA polymerase.

    Blend Taq® and Blend Taq® -Plus- are highly efficient Taq-based DNA polymerases developed based on the Barns' method. This method uses a DNA polymerase which lacked 3'→5' exonuclease (proofreading) activity (e.g., Taq DNA polymerase) and a small amount of an archaeal DNA polymerase with proofreading activity. Because the proofreading activity repairs misincorporated nucleotide bases causing the termination of the polymerase reaction, PCR with a ‘mixed' enzyme solution enables highly efficient amplification. The enzyme solution of Blend Taq® -Plus- contains anti-Taq DNA polymerase antibodies that inhibit polymerase activity, allowing for Hot Start PCR. Blend Taq® and Blend Taq® -Plus- generate dA overhang-ended PCR products. Therefore, the PCR products can be cloned using a standard TA-cloning method.

    Code No. BTQ-101 250 U 200 reactions <Blend Taq®>

    Code No. BTQ-201 250 U 200 reactions <Blend Taq® -Plus->

     

    PCR related product

     

    Anti-Taq DNA polymerase antibody

    Anti-Taq high

    This product is a highly purified neutralization monoclonal antibody to Taq DNA polymerase. The product can be applied for hot start PCR using Taq DNA polymerase by mixing with the Taq DNA polymerase solution.

    Code No. TCP-101 100μl

     

     

    dNTPs Mixture(2mM)

    dNTPs Mixture(10mM)

    • 2 mM and 10 mM solutions are available
    • Suitable for PCR and reverse transcriptation

    dNTPs Mixture is an equal moler solution mixture of ultrapure dATP, dCTP, dGTP and dTTP.

    Code No. NTP-201 2 µmoles/1 ml (2 mM)

    Code No. NTP-301 2 µmoles/0.2 ml (10 mM)

     

    RNase inhibitor

    This product is a recombinant type human ribonuclease inhibitor (RNase inhibitor; 51kDa) that is produced by E. coli. RNase inhibitor forms a complex with ribonuclease A (RNase A) and inhibits the activity of RNase A. This inhibitor can be applied to reverse transcriptase reactions.

    Code No. SIN-201 2,500 U

     

     

     

    High efficient TA cloning kit

    TArget Clone™

    • Ligation step can be completed in 5 min with high efficiency.
    • PCR products can be used without purification.

    TArget Clone™ [Code No. TAK-101] can be applied to the TA cloning of PCR products amplified using Taq DNA polymerase, Blend Taq® [Code No. BTQ-101], Blend Taq® -Plus- [Code No. BTQ-201] or KOD Dash [Code No. LDP-101]. Almost all PCR products having dA overhanging at the 3'end are available.

    Code No. TAK-101 10 reactions

     

     

    High efficient TA cloning kit for KOD products

    TArget Clone™ -Plus-

    • Can be applied to the TA cloning of blunt-end PCR products of KOD DNA polymerase.
    • Ligation step can be completed in 5 min with high efficiency.
    • PCR products can be used without purification.

    TArget Clone™ -Plus- [Code No. TAK-201] can be applied to the TA cloning of blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201, KOD-401] or KOD FX [Code No. KFX-101, KFX-201]. TArget Clone™ -Plus- contains 10xA-attachment mix. This reagent is a mixture of anti-KOD DNA polymerase antibody specific to KOD 3'→5' exonuclease activity (proof-reading activity) and Taq DNA polymerase, which exhibits terminal transferase activity. The 10 x A-attachment mix allows for blunt end PCR products to acquire overhanging dA at the 3'-ends

    Code No. TAK-201 10 reactions

     

     

    dA attachment reagent

    10xA-attachment mix

    • The attachment reaction is completed in 10 minutes at 60ºC.
    • The cloning efficiency of dA attached PCR products is as great as that of PCR products of Taq DNA polymerase.

    10 x A-attachment mix is a reagent consists of the anti-KOD DNA polymerase antibody for the 3'→5' exonuclease activity (proof-reading activity) of the enzyme, and Taq DNA polymerase exhibits the terminal transferase activity. The PCR products from KOD -Plus- [Code No. KOD-201] and KOD FX [Code No. KFX-101] have blunt ends due to the 3'→5' exonuclease activity of KOD DNA polymerase. 10 x A-attachment mix allows the PCR products to acquire overhanging dA at the 3'-ends. The products having 3'-dA overhang can be directly cloned into arbitrary T-vectors using ligation reagents such as Ligation high Ver.2 [Code No. LGK-201].

    Code No. TAK-301 25 reactions

     

     

     

  • Specifications:

    Toyobo has various real-time PCR reagents showing below. The following table shows the characteristics of each product. The detailed information can be referred in the linked sites.

    Enzyme Product Name Assay type One-step RT-PCR Hot start (Antibody) Passive reference Glass capillary Specificity Efficiency Long target amplification GC-rich targets Amplification from crude samples
    Taq DNA polymerase Realtime PCR Master Mix Probe   check check check +++ ++ + + +
    SYBR® Green Realtime PCR Master Mix (-Plus-) SYBR®   check check check + ++ + + +
    TUNDERBIRD® Probe qPCR Mix Probe   check check check +++ ++ + + +
    TUNDERBIRD® SYBR® qPCR Mix SYBR®   check check check ++ ++ + + +
    Tth DNA polymerase [One Step] RNA-direct™ Realtime PCR Master Mix Probe check check check check +++ ++ + ++ ++
    RNA-direct™ SYBR® Green Realtime PCR Master Mix SYBR® check check check check ++ ++ + ++ ++
    KOD exo(-) DNA polymerase KOD SYBR® qPCR Mix SYBR®   check check check ++ ++ +++
    (<2 kb)
    +++ +++

    +++: BEST, ++: Excellent, +: Good, : Applicable

  • Specifications:

    Toyobo has various kits for cDNA synthesis based on the genetically improved M-MLV reverse transcriptase "ReverTra Ace®". ReverTra Ace -α-® is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®", as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR.
    ReverTra Ace® qPCR RT Kit (Code No. FSQ-101) is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase "ReverTra Ace®" and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. ReverTra Ace® qPCR Master Mix (Code No. FSQ-201) is a premix version of the ReverTra Ace® qPCR RT Kit.
    ReverTra Ace® qPCR RT Master Mix with gDNA remover (Code No. FSQ-301) is an efficient and convenient kit, consisting of master mix reagents, to synthesize high quality cDNAs for real-time PCR. The kit includes reagents for reverse transcription and for the removal of genomic DNA. The protocol consists of i) a genomic DNA degradation step using "gDNA remover [DNase I]" and ii) a reverse transcription step. The two steps can be achieved sequentially without purification or heat inactivation of DNase I.

     

    cDNA synthesis Kits

    Enzyme Product Name Application Efficiency Long cDNA
    synthesis
    Master Mix Genomic DNA
    removal
    Recommend PCR enzymes
    ReverTra
    Ace®
    ReverTra Ace -α-® Conventional
    RT-PCR
    + ++
    (> 10 kb)
        KOD –Plus- series
    KOD FX series
    Taq-based PCR reagent
    ReverTra Ace® qPCR RT Kit qPCR ++ +
    (< 5 kb)
        THUNDERBIRD® series
    Realtime PCR Master Mix series
    KOD SYBR® qPCR Mix
    KOD –Plus- series
    KOD FX series
    Taq-based PCR reagent
    ReverTra Ace® qPCR RT Master Mix qPCR ++ +
    (< 5 kb)
     
    ReverTra Ace® qPCR RT Master Mix with gDNA Remover qPCR ++ +
    (< 5 kb)
    SuperPrep® Cell Lysis & RT Kit for qPCR qPCR from
    cultured cells
    ++ +
    (< 5 kb)

    ++: Excellent, +: Good, : Applicable

  • Specifications:

    Toyobo has various modifying enzymes. The detailed information can be referred in the following linked sites.

    Applicatins Product Name
    High efficient reverse transcription ReverTra Ace®
    Stable transcription (T7 promoter) Thermo T7 RNA polymerase
    Substrate for reverse transcription dNTPs Mixture
    Dephosphorylation of DNA fragments Bacterial Alkaline Phosphatase
    Dephosphorylation of DNA fragments Calf intestine Alkaline Phosphatase
    Kination of DNA fragments T4 Polynucleotide Kinase
  • Specifications:

    The detailed information can be referred in the following linked sites.

    Applications Product Name
    DNA fragment purification MagExtractor -PCR & Gel Clean up-
    High efficient ligation Ligation high Ver.2
    High efficient blunting Blunting high
    TA cloning of PCR products having overhanged dA at the 3'-ends.
    PCR products from Taq DNA polymerase, Blend Taq [Code No. BTQ-101],
    Blend Taq -Plus- [Code No. BTQ-201] or KOD Dash [Code No. LDP-101]
    are available.
    TArget Clone
    TA cloning of blunt-end PCR products amplified using
    KOD -Plus- [Code No. KOD-201] or KOD FX [Code No. KFX-101].
    TArget Clone -Plus-
    Attachment of dA at the 3' - ends of blunt end PCR products
    from KOD -Plus- and KOD FX ( For TA cloning )
    10xA-attachment mix
    Site-directed mutagenesis KOD -Plus- Mutagenesis Kit
    Southern & Northern hybridization PerfectHyb hybridization solution
    100 bp DNA Ladder Marker 100 bp DNA Ladder
    1 kb DNA Ladder Marker 1 kb DNA Ladder
  • Specifications:

    Toyobo has various nucleic acid purification kits using magnetic beads as shown below. The following table shows the applications of each product. Detailed information can be obtained by going to the linked sites.

    Product Name Sample Nucleic acid
    MagExtractor -Genome- Whole blood, Cultured cell Genomic DNA
    MagExtractor -Plant Genome- Plant tissue, Cultured plant cell Genomic DNA
    MagExtractor -RNA- Cultured cell, tissue Total RNA
    MagExtractor -Viral RNA- Serum, Plasma Viral RNA
    MagExtractor -Plasmid- E. coli cell Plasmid
    MagExtractor -PCR & Gel Clean up- PCR solution, Enzyme treated solution, Agarose gel DNA fragment
    Magical Trapper Magnetic stand
  • Specifications:

    The detailed information can be referred in the following linked sites.

    Applications Product Name
    Enhancement of Western blot & ELISA Can Get Signal®
    Efficient Blocking Buffer PVDF Blocking Reagent for Can Get Signal®
    Enhancement of IHC Can Get Signal® immunostain
  • Specifications:

    Catalog

Description: 
Toyobo was founded in 1882 as a textile company, when it began its spinning and textile business. We continued to adapt to the changing needs of the times, drawing on our core technologies in polymerization, modification, processing and biotechnology to expand our business fields and develop the kind of high-performance products. The Toyobo Group seeks to become a manufacturer of high-function products, continuously creating new value by enhancing their core technologies. We distribute TOYOBO's Bioproducts for Life Science. These include High performance enzymes that include high success-rate, high fidelity and high efficiency DNA polymerases (KOD DNA polymerases), and reagents for Site-directed mutagenesis. Other reagents include enzymes and reagents for molecular biology applications. Catalog: www.axilscientific.com/downloads/products/Toyobo/TOYOBO-Catalog2016-2017-AXIL.pdf
Country: 
Japan