RNA Extraction for COVID-19

 

Rapid RNA Extraction in 3 minutes

Rapid RNA extraction is critical in timely delivery of SARS-CoV-2 (COVID-19) testing. Arcis enables users to go from biological sample to downstream testing in under 3 minutes, with a simple and highly automation-compatible workflow. Current pilot project with Hospital Carlos III & CIBERehd (Madrid, Spain) have further demonstrated compatibility with SARS-CoV-2 qPCR assay.

  • Reduce RNA extraction times to 3 min faster clinical decision making
  • Inactivates pathogens decrease risk for healthcare workers
  • Compatible with liquid handling decrease risk for laboratory staff & support high-throughput testing
  • Compatible with qPCR testing of coronavirus ready for use

Extraction protocols compatible with:

  1. Swabs in Viral Transport Medium
  2. Sputum / Saliva Samples
  3. Swabs without Transport Medium

Protocol https://arcisbio.com/wp-content/uploads/2020/03/COVID-19-Protocols_v1.pdf

Products

Product Cat. No Volume
Arcis Sample Prep Kit UFL002 50 rxns
Arcis Pathogen Kit (IVD) UFL004 50 rxns
Arcis Coronavirus RNA extraction research kit SCV019 50 rxns

Contact us to optimise the process for use in your workflow!

 

-----

Geneaid

Geneaid Viral Nucleic Acid Extraction Kit II 

The quality of Viral Nucleic Acid Extraction Kit II is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample. 

  • High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number! 
  • Purify virus DNA or virus RNA in 20 minutes! 
  • Sample: up to 200 μl plasma, serum, body fluid or the supernatant of viral infected cell cultures 
  • Elution Volume: 50 μl 

Viral Nucleic Acid Extraction Kit II Functional Test Data 


Detection System (3 replications of each copy number). Serum samples containing various amounts of DNA/RNA viruses ranging from 10E1 to 10E6 copies/ml were successfully detected and identified. The low Ct values indicate a high number of target nucleic acid in the sample.

 

Product Cat. No. Preps
Viral Nucleic Acid Extraction Kit II 

VR050 

50 preps 
VR100 100 preps 
VR300 300 preps

 

Magnetic Beads Virus DNA/RNA Extraction Kit 

  • High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number! 
  • Easily adapted to automated magnetic bead separation instruments and workstations, eg Hamilton, Kingfisher
  • Magnetic Bead Concentration: 50 mg/ml 
  • Magnetic Bead Size: ~ 5 µm 
  • Sample: up to 200 µl of virus samples (plasma, serum, body fluid or the supernatant of viral infected cell cultures)  
  • Manual or automated DNA/RNA isolation 
  • Operation time: within 30 minutes (manual) 

Magnetic Beads Virus DNA/RNA Extraction Kit Functional Test Data 

Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the Geneaid Magnetic Beads Virus DNA/RNA Extraction Kit (2 replications of each copy number). The purified RNA was eluted with 50 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 5 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV.

Product Cat. No. Preps
Magnetic Beads Virus DNA/RNA Extraction Kit 

MV048

48 preps 
MV096 96 preps 

 

-----

Simplified & Efficient DNA/RNA Extraction from Viral Samples

HighPrep Viral DNA/RNA Kit – DNA & RNA Extraction

  • Rapid and reliable extraction from whole blood, serum, plasma, viral samples and other body fluids.
  • Time efficient protocol allowing extraction of high-quality DNA/RNA suitable for direct use in downstream applications.
  • Yields highly concentrated viral nucleic acids for greater sensitivity in detection.
  • High sensitivity for best detection of low-copy viruses.
  • Adaptable to various automated liquid handling workstations, eg Hamilton, Kingfisher

For protocol, click here.

Product Cat. No. Preps
HighPrep™ Viral DNA/RNA Kit HPV-DR96 96 preps
HPV-DR96x4 384 preps