RNA Extraction for COVID-19

​Rapid RNA Extraction in 3 minutes

Rapid RNA extraction is critical in timely delivery of SARS-CoV-2 (COVID-19) testing. Arcis enables users to go from biological sample to downstream testing in under 3 minutes, with a simple and highly automation-compatible workflow. Current pilot project with Hospital Carlos III & CIBERehd (Madrid, Spain) have further demonstrated compatibility with SARS-CoV-2 qPCR assay.

• Reduce RNA extraction times to 3 min faster clinical decision making
• Inactivates pathogens decrease risk for healthcare workers
• Compatible with liquid handling decrease risk for laboratory staff & support high-throughput testing
• Compatible with qPCR testing of coronavirus ready for use

Extraction protocols compatible with:

1. Swabs in Viral Transport Medium
2. Sputum / Saliva Samples
3. Swabs without Transport Medium

Protocol https://arcisbio.com/wp-content/uploads/2020/03/COVID-19-Protocols_v1.pdf

Products

Contact us to optimise the process for use in your workflow!

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Geneaid Viral Nucleic Acid Extraction Kit II 

The quality of Viral Nucleic Acid Extraction Kit II is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample. 

• High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number! 
• Purify virus DNA or virus RNA in 20 minutes! 
• Sample: up to 200 μl plasma, serum, body fluid or the supernatant of viral infected cell cultures 
• Elution Volume: 50 μl 

Viral Nucleic Acid Extraction Kit II Functional Test Data 

Detection System (3 replications of each copy number). Serum samples containing various amounts of DNA/RNA viruses ranging from 10E1 to 10E6 copies/ml were successfully detected and identified. The low Ct values indicate a high number of target nucleic acid in the sample.

Magnetic Beads Virus DNA/RNA Extraction Kit 

• High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number! 
• Easily adapted to automated magnetic bead separation instruments and workstations, eg Hamilton, Kingfisher
• Magnetic Bead Concentration: 50 mg/ml 
• Magnetic Bead Size: ~ 5 µm 
• Sample: up to 200 µl of virus samples (plasma, serum, body fluid or the supernatant of viral infected cell cultures)  
• Manual or automated DNA/RNA isolation 
• Operation time: within 30 minutes (manual) 

Magnetic Beads Virus DNA/RNA Extraction Kit Functional Test Data 

^{Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the Geneaid Magnetic Beads Virus DNA/RNA Extraction Kit (2 replications of each copy number). The purified RNA was eluted with 50 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 5 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV.}

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Simplified & Efficient DNA/RNA Extraction from Viral Samples

HighPrep Viral DNA/RNA Kit – DNA & RNA Extraction

• Rapid and reliable extraction from whole blood, serum, plasma, viral samples and other body fluids.
• Time efficient protocol allowing extraction of high-quality DNA/RNA suitable for direct use in downstream applications.
• Yields highly concentrated viral nucleic acids for greater sensitivity in detection.
• High sensitivity for best detection of low-copy viruses.
• Adaptable to various automated liquid handling workstations, eg Hamilton, Kingfisher

For protocol, click here.