RT-qPCR Reagents for COVID-19
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We offer various PCR reagents to cater to the different needs for any stage of SARS-CoV-2 assay development, infection mechanism study or vaccine research.
[Research Use] SENSIFAST Probe One-Step for FAST . SENSITIVE . REPRODUCIBLE Results
The SensiFAST™ Probe One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube and uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. This also gives SensiFAST Probe One-Step unbeatable efficiency in multiplexing.
- Novel 1-step buffer optimized for highly-efficient RT and PCR steps
- Improved consistency between technical replicates
- Reproducible, accurate results in as little as 40 minutes
- Antibody-mediated hot-start minimizes non-specific amplification
- Excellent efficiency for improved multiplexing
Products
Product | Cat No. | Reactions |
SensiFAST™ Probe No-ROX One-Step Kits | BIO-76005 | 500 rxns |
BIO-76001 | 100 rxns | |
BIO-78005 | 500 rxns | |
SensiFAST™ Probe Lo-ROX One-Step Kits | BIO-78001 | 100 rxns |
SensiFAST™ Probe Hi-ROX One-Step Kits | BIO-77005 | 500 rxns |
BIO-77001 | 100 rxns |
The SensiFAST Probe One-Step Kit (red) gaves the lowest Ct values while maintaining reproducibility of technical replicates as compared to Supplier A (green) and Q (blue).
RT-PCR analysis of a mixed population of influenza viruses in ferret nasal wash to measure the viral replication and transmission kinetics of each virus population.
[Molecular Assay Development] Novel RT-qPCR Reagent Solutions for Fast and Accurate Screening Assays
Diagnostic companies and laboratories in China have turned to Meridian’s Lyo-Ready 1-Step RT-qPCR Mix to develop fast and accurate screening assays for 2019-nCoV in response to the outbreak.
Fast 1-Step RT-qPCR Mix
- Formulated for fast, automated, high-throughput systems
- Sensitive detection of low-copy number DNA and RNA targets
- Highly suited to multiplex assays from a broad range of sample types
Lyo-Ready 1-Step RT-qPCR Mixes
- Provided glycerol-free and pre-formulated with a specialized blend of lyo-excipients
- Virus mix (Cat #MDX062) is specifically optimized for amplification of RNA or DNA viruses with a high secondary structure (reverse transcriptase remains active at 55-60 °C)
- Suited for multiplex-assays and low-copy number targets • Compatible with lyophilization into beads or cakes
- Ideal for developing fast, highly reproducible assays requiring ambient temperature transport and storage
Products
Product | Cat. No | Volume | Reactions |
Fast 1-Step RT-qPCR Mix | MDX032 | 10 ml | 1,000 reactions |
100 ml | 10,000 reactions | ||
Lyo-Ready 1-Step RT-qPCR Mix |
MDX024 | 10 ml | 1,000 reactions |
100 ml | 10,000 reactions | ||
Lyo-Ready 1-Step RT-qPCR Virus Mix |
MDX062 | 10 ml | 1,000 reactions |
100 ml | 10,000 reactions |
The results demonstrate the ability of Lyo-Ready 1-Step RT-qPCR Mix (Cat #MDX024) (purple traces) to amplify from difficult samples such as blood, in contrast to the competitor mix (green traces) which fail, even with higher concentrations of the template RNA.
RT-PCR analysis of a mixed population of influenza viruses in ferret nasal wash to measure the viral replication and transmission kinetics of each virus population.
Visit link below for Meridian's complete solutions for COVID-19 diagnostics:
axilscientific.com/product/meridian-life-science/complete-solutions-covid-19-diagnostics
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Japan NIID Recommended Reagents
There are two methods established by National Institute of Infectious Diseases (NIID), qualitative detection by nested PCR method and alternatively, real-time one-step probe based RT-qPCR method.
Quick Taq® HS Dyemix (Code No. DTM-101) was used to perform the nested PCR outline in the published manual.
- Enables greater PCR performance than conventional Taq DNA polymerase.
- anti-Taq antibodies for hot start PCR. Hot start technology realizes highly specific and sensitive PCR
For more information, click here.
THUNDERBIRD ® Probe One-step qRT-PCR Kit (QRZ-101) for one-step rt-qPCR method.
- Rapid and highly sensitive
- Tolerant of target sequence diversity
- Tolerant of PCR inhibitors
- Utilization of dUTP to avoid carry over contamination
- Multiplex detection
For more information, click here.
For published manual, click here.
For NIID published manual, click here.
A 4n dilution series of various viral RNAs was detected. The primers and TaqMan® probes were synthesized in accordance with previous reports. The graph indicates the minimum copy numbers that were detected by the kits. THUNDERBIRD® Probe One-step qRT-PCR Kit was the only kit that detected all viral RNAs tested at high sensitivity (≤30 copies)
Product
Product | Cat. No | Reactions |
Quick Taq ® HS DyeMix | DTM-101 | 100 rxns (50ul rxn volume) |
THUNDERBIRD ® Probe One-step qRT-PCR Kit | QRZ-101 | 250 rxns (20ul rxn volume) |
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Other Relevant Products
Reduce chance of obtaining a false negative result in the sample RNA by
- Determine if there are inhibitors in the PCR assay
- Validate the success of the extraction step
Product | Cat. No | Reactions |
RT-qPCR Extraction Control Red | MDX028 | 500 rxns |
RT-qPCR Extraction Control Orange | MDX029 | 500 rxns |
Molecular Grade Water
Product | Cat. No | Reactions |
Water, Biotechnology Grade |
BUF-1180-100ml | 100ml |
BUF-1180-500ml | 500ml | |
BUF-1180-1L | 1L |