RT-qPCR Reagents for COVID-19

We offer various PCR reagents to cater to the different needs for any stage of SARS-CoV-2 assay development, infection mechanism study or vaccine research.

[Research Use] SENSIFAST Probe One-Step for FAST . SENSITIVE . REPRODUCIBLE Results

The SensiFAST™ Probe One-Step Kits have been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube and uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. This also gives SensiFAST Probe One-Step unbeatable efficiency in multiplexing.

• Novel 1-step buffer optimized for highly-efficient RT and PCR steps
• Improved consistency between technical replicates
• Reproducible, accurate results in as little as 40 minutes
• Antibody-mediated hot-start minimizes non-specific amplification
• Excellent efficiency for improved multiplexing

Products

^{The SensiFAST Probe One-Step Kit (red) gaves the lowest Ct values while maintaining reproducibility of technical replicates as compared to Supplier A (green) and Q (blue).}

^{RT-PCR analysis of a mixed population of influenza viruses in ferret nasal wash to measure the viral replication and transmission kinetics of each virus population.}

[Molecular Assay Development] Novel RT-qPCR Reagent Solutions for Fast and Accurate Screening Assays

Diagnostic companies and laboratories in China have turned to Meridian’s Lyo-Ready 1-Step RT-qPCR Mix to develop fast and accurate screening assays for 2019-nCoV in response to the outbreak.

Fast 1-Step RT-qPCR Mix

• Formulated for fast, automated, high-throughput systems
• Sensitive detection of low-copy number DNA and RNA targets
• Highly suited to multiplex assays from a broad range of sample types

Lyo-Ready 1-Step RT-qPCR Mixes

• Provided glycerol-free and pre-formulated with a specialized blend of lyo-excipients
• Virus mix (Cat #MDX062) is specifically optimized for amplification of RNA or DNA viruses with a high secondary structure (reverse transcriptase remains active at 55-60 °C)
• Suited for multiplex-assays and low-copy number targets • Compatible with lyophilization into beads or cakes
• Ideal for developing fast, highly reproducible assays requiring ambient temperature transport and storage

Products

^{The results demonstrate the ability of Lyo-Ready 1-Step RT-qPCR Mix (Cat #MDX024) (purple traces) to amplify from difficult samples such as blood, in contrast to the competitor mix (green traces) which fail, even with higher concentrations of the template RNA.}

^{RT-PCR analysis of a mixed population of influenza viruses in ferret nasal wash to measure the viral replication and transmission kinetics of each virus population.}

Visit link below for Meridian's complete solutions for COVID-19 diagnostics:
axilscientific.com/product/meridian-life-science/complete-solutions-covid-19-diagnostics

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Japan NIID Recommended Reagents

There are two methods established by National Institute of Infectious Diseases (NIID), qualitative detection by nested PCR method and alternatively, real-time one-step probe based RT-qPCR method.

Quick Taq® HS Dyemix (Code No. DTM-101) was used to perform the nested PCR outline in the published manual.

• Enables greater PCR performance than conventional Taq DNA polymerase.
• anti-Taq antibodies for hot start PCR. Hot start technology realizes highly specific and sensitive PCR

For more information, click here. 

THUNDERBIRD ® Probe One-step qRT-PCR Kit (QRZ-101) for one-step rt-qPCR method.

• Rapid and highly sensitive
• Tolerant of target sequence diversity
• Tolerant of PCR inhibitors
• Utilization of dUTP to avoid carry over contamination
• Multiplex detection

For more information, click here. 

For published manual, click here.

For NIID published manual, click here. 

^{A 4n dilution series of various viral RNAs was detected. The primers and TaqMan® probes were synthesized in accordance with previous reports. The graph indicates the minimum copy numbers that were detected by the kits. THUNDERBIRD® Probe One-step qRT-PCR Kit was the only kit that detected all viral RNAs tested at high sensitivity (≤30 copies)}

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Other Relevant Products

Extraction Control 

Reduce chance of obtaining a false negative result in the sample RNA by

• Determine if there are inhibitors in the PCR assay
• Validate the success of the extraction step

Molecular Grade Water